Function discovery of a non-ribosomal peptide synthetase-like encoding gene in the nematode-trapping fungus Arthrobotrys oligospora

In this study, the function of a non-ribosomal peptide synthetase-like (NRPS-like) encoding gene AOL_s00188g306 ( g306 ) was investigated to reveal association between NRPS and nematocidal activity in nematode-trapping fungus Arthrobotrys oligospora . Sequence analysis indicated that encoded product is an adenylation domain synthetases extended short-chain dehydrogenase/reductase domain-containing proteins, displays wide substrate spectrum. The Δ mutants were more sensitive chemical stressors than wild type. Disruption impeded efficiency A. Metabolomics showed secondary metabolite biosynthesis lipid metabolism altered mutants. phenotypic changes can be attributed down-regulation various metabolites, including fatty acyls, prenol lipids, steroidsand steroid derivative, amino acid derivatives, identified present study. This study polypeptide-encoding nematicidal , providing reference for resolving predation mechanism fungus.